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人Salusin肽β(SALb)酶联免疫吸附检测试剂盒
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ELK10326
规格: 价格:
48T ¥1820.00
96T ¥2600.00
96T*5 ¥11050.00

Overview

产品名称: Human SALb(Salusin Beta) ELISA Kit
反应性: Human
别名: Salusin Beta
反应类型: Competitive Inhibition
灵敏度: 0.056 ng/mL
标准品: 10 ng/mL
检测范围: 0.16-10 ng/mL
样本类型: serum, plasma and other biological fluids
反应时长: 2h
研究领域: Metabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;
检测原理: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human SALb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SALb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SALb in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL) OD Corrected OD
10.00 0.199
5.00 0.396
2.50 0.557
1.25 0.861
0.63 1.119
0.32 1.565
0.16 1.799
0.00 2.115

精密度

板内精密度(测定内的精密度):CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次,以评估分析板内精密度。

板间精密度(测定板间间精密度):CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次,以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的SALb, 做回收率实验,得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 86-99% 92%
EDTA 血浆(n=5) 79-93% 86%
Heparin 血浆(n=5) 90-105% 97%

线性

将添加有SALb的样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 95-106% 87-96% 82-90% 92-101%
EDTA 血浆(n=5) 85-92% 95-103% 97-105% 93-102%
Heparin 血浆(n=5) 88-97% 97-105% 81-93% 89-102%
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