人血小板反应蛋白1(TSP-1)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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人血小板反应蛋白1(TSP-1)酶联免疫吸附检测试剂盒

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ELK10311

规格：	价格：
48T	￥1820.00
96T	￥2600.00
96T*5	￥11050.00

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Overview 文献(2)

Product name:	Human TSP-1(Thrombospondin-1) ELISA Kit
Reactivity:	Human
Alternative Names:	TSP1; Thrombospondin-1p180; THBS1; Thrombospondin 1
Assay Type:	Sandwich
Sensitivity:	0.47 ng/mL
Standard:	100 ng/mL
Detection Range:	1.56-100 ng/mL
Sample Type:	plasma
Assay Length:	3.5h
Research Area:	Infection immunity;Hematology;
Uniprot ID:	P07996
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TSP-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TSP-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TSP-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TSP-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Correlation of Lp(a), ApoB and oxLDL with Endothelial Damage Reading in Patients with Different Degrees of Coronary Atherosclerosis

日期：2026-01-19 IF：4.9
World Journal of Experimental Medicine

Clinical significance of differential plasma proteins levels in the diagnosis of epithelial ovarian cancer

日期：2025-09-20 IF：

标准曲线

Concentration (ng/mL)	OD	Corrected OD
100.00	2.164	2.040
50.00	1.782	1.658
25.00	1.205	1.081
12.50	0.963	0.839
6.25	0.547	0.423
3.13	0.339	0.215
1.57	0.227	0.103
0.00	0.124	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant TSP-1 and the recovery rates were calculated by comparing the measured value to the expected amount of TSP-1 in samples.

Matrix	Recovery range	Average
serum(n=5)	85-99%	92%
EDTA plasma(n=5)	93-107%	100%
Heparin plasma(n=5)	87-99%	93%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TSP-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.