小鼠高迁移率族蛋白B1(HMGB-1)微量上样酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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小鼠高迁移率族蛋白B1(HMGB-1)微量上样酶联免疫吸附检测试剂盒

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ELK10273MS

规格：	价格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下载说明 ①

Overview

Product name:	Mouse HMGB-1(High Mobility Group Protein B1) Microsample ELISA Kit
Reactivity:	Mouse
Alternative Names:	HMGB1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein; HMG1; High Mobility Group Protein 1
Assay Type:	Sandwich
Sensitivity:	0.063 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	serum, plasma and other biological fluids
Assay Length:	3.5h
Research Area:	Infection immunity;
Uniprot ID:	P63158
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HMGB-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HMGB-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HMGB-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HMGB-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
10.00	2.219	2.153
5.00	1.575	1.509
2.50	1.210	1.144
1.25	0.754	0.688
0.63	0.556	0.490
0.31	0.333	0.267
0.16	0.225	0.159
0.00	0.066	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant HMGB-1 and the recovery rates were calculated by comparing the measured value to the expected amount of HMGB-1 in samples.

Matrix	Recovery range	Average
serum(n=5)	83-95%	89%
EDTA plasma(n=5)	82-124%	103%
Heparin plasma(n=5)	89-119%	104%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HMGB-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.