大鼠高敏C反应蛋白(hs-CRP)微量上样酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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大鼠高敏C反应蛋白(hs-CRP)微量上样酶联免疫吸附检测试剂盒

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ELK10185MS

规格：	价格：
48T	￥1680.00
96T	￥2400.00
96T*5	￥10200.00

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Overview

Product name:	Rat hs-CRP(High Sensitivity C Reactive Protein) Microsample ELISA Kit
Reactivity:	Rat
Alternative Names:	C-RP; PTX1; Pentraxin-Related; Pentraxin 1
Assay Type:	Sandwich
Sensitivity:	1.08 µg/mL
Standard:	100 µg/mL
Detection Range:	1.56-100 µg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Tumor immunity;Infection immunity;Cardiovascular biology;Rheumatology;
Uniprot ID:	P48199
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat hs-CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat hs-CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat hs-CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat hs-CRP in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (µg/mL)	OD	Corrected OD
100.00	1.770	1.674
50.00	1.285	1.189
25.00	0.810	0.714
12.50	0.561	0.465
6.25	0.415	0.319
3.13	0.380	0.284
1.56	0.211	0.115
0.00	0.096	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant hs-CRP and the recovery rates were calculated by comparing the measured value to the expected amount of hs-CRP in samples.

Matrix	Recovery range	Average
serum(n=5)	80-92%	86%
EDTA plasma(n=5)	84-112%	98%
Heparin plasma(n=5)	90-117%	104%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of hs-CRP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.