人抗21-羟化酶抗体(21-OH Ab)酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子、生化试剂等全球供应商

一键复制产品信息

ELK10071

下载说明 ①

产品名称:	Human anti-21-OH Ab(anti 21-hydroxylase antibody) ELISA Kit
反应性:	Human
别名:	21-OH Ab
反应类型:	Sandwich
灵敏度:	0.189 ng/mL
标准品:	20 ng/mL
检测范围:	0.32-20 ng/mL
样本类型:	serum, plasma,Tissue homogenates and other biological fluids.
反应时长:	3.5h
研究领域:	Immunology
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 21-OH Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 21-OH Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 21-OH Ab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 21-OH Ab in the samples is then determined by comparing the OD of the samples to the standard curve.