转化生长因子β1(TGF-b1)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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转化生长因子β1(TGF-b1)酶联免疫吸附检测试剂盒

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ELK10034

规格：	价格：
48T	￥1960.00
96T	￥2800.00
96T*5	￥11900.00

下载说明 ①

Overview

Product name:	TGF-b1(TransformingGrowth Factor Beta 1) ELISA Kit
Reactivity:	General
Alternative Names:	Cartilage-inducing factor,CED,Differentiation inhibiting factor,DPD1,LAP,Latency-associated peptide,Prepro transforming growth factor beta 1,TGF beta 1,TGF beta,TGF beta 1 protein,TGF-beta 1 protein,TGF-beta-1,TGF-beta-5,TGF-beta1,TGFB,Tgfb-1,tgfb1,TGFB1,TGFbeta,TGFbeta1,Transforming Growth Factor b1,Transforming Growth Factor beta 1,Transforming growth factor beta 1a,transforming growth factor beta-1,transforming growth factor,beta 1,Transforming Growth Factor-ß1
Assay Type:	Sandwich
Sensitivity:	0.11 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	Activated serum, plasma
Assay Length:	3.5h
Research Area:	Cytokine;Tumor immunity;Infection immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGF-b1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TGF-b1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TGF-b1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TGF-b1 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
10.00	2.258	2.170
5.00	1.593	1.505
2.50	1.136	1.048
1.25	0.847	0.759
0.63	0.518	0.430
0.32	0.385	0.297
0.16	0.226	0.138
0.00	0.088	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant TGF-b1 and the recovery rates were calculated by comparing the measured value to the expected amount of TGF-b1 in samples.

Matrix	Recovery range	Average
serum(n=5)	82-95%	88%
EDTA plasma(n=5)	87-101%	94%
Heparin plasma(n=5)	87-99%	93%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TGF-b1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.