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大鼠磷酸化腺苷酸活化蛋白激酶(p-AMPK)酶联免疫吸附检测试剂盒
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ELK0962
规格： 价格：
48T ￥1820.00
96T ￥2600.00
96T*5 ￥11050.00
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产品名称: Rat p-AMPK(Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit
反应性: Rat
别名: p-PRKAA1; p-AMPK; p-AMPKa1; p-AMPK alpha 1
反应类型: Sandwich
灵敏度: 1.25 ng/mL
标准品: 200 ng/mL
检测范围: 3.13-200 ng/mL
样本类型: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
反应时长: 3.5h
研究领域: Enzyme & Kinase
检测原理: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat p-AMPK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat p-AMPK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat p-AMPK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat p-AMPK in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL) OD Corrected OD
200.00 2.266 2.172
100.00 1.686 1.592
50.00 1.223 1.129
25.00 0.828 0.734
12.50 0.567 0.473
6.25 0.336 0.242
3.13 0.256 0.162
0.00 0.094 0.000

精密度

板内精密度（测定内的精密度）：CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次，以评估分析板内精密度。

板间精密度（测定板间间精密度）：CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次，以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的p-AMPK， 做回收率实验，得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 83-98% 90%
EDTA 血浆(n=5) 90-105% 97%
Heparin 血浆(n=5) 87-95% 91%

线性

将添加有p-AMPK的样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 79-92% 87-103% 89-98% 80-101%
EDTA 血浆(n=5) 89-96% 90-99% 85-96% 82-93%
Heparin 血浆(n=5) 91-105% 87-98% 99-102% 89-102%
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