一步法人白介素1受体Ⅱ(IL1R2)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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一步法人白介素1受体Ⅱ(IL1R2)酶联免疫吸附检测试剂盒

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ELK090ES

规格：	价格：
48T	￥2240.00
96T	￥3200.00
96T*5	￥13600.00

下载说明 ①

Overview

Product name:	EasyStep Human IL1R2(Interleukin 1 Receptor Type II) ELISA Kit
Reactivity:	Human
Alternative Names:	IL-1R2；CD121b; CD121-B; IL1-R2; IL1RB; Interleukin-1 Receptor Beta; CD121 Antigen-like Family Member
Assay Type:	Sandwich
Sensitivity:	12.7 pg/mL
Standard:	5000 pg/mL
Detection Range:	78.13-5000 pg/mL
Sample Type:	Serum, plasma
Assay Length:	1.5h
Research Area:	Signal transduction;CD & Adhesion molecule;Infection immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL1R2, and the Human IL1R2 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL1R2. After TMB substrate solution is added, only those wells that contain Human IL1R2 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL1R2 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
5000.00	2.168	2.084
2500.00	1.536	1.452
1250.00	1.182	1.098
625.00	0.928	0.844
312.50	0.473	0.389
156.25	0.297	0.213
78.13	0.189	0.105
0.00	0.084	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IL1R2 and the recovery rates were calculated by comparing the measured value to the expected amount of IL1R2 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	83-98%	90%
Heparin plasma(n=5)	86-99%	92%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL1R2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.