人磷酸二酯酶4A(PDE4A)酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子、生化试剂等全球供应商

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ELK0902

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产品名称:	Human PDE4A(Phosphodiesterase 4A, cAMP Specific) ELISA Kit
反应性:	Human
别名:	PDE4; DPDE2; PDE46
反应类型:	Sandwich
灵敏度:	26.7 pg/mL
标准品:	4000 pg/mL
检测范围:	62.5-4000 pg/mL
样本类型:	Serum, plasma, tissue homogenates and other biological fluids.
反应时长:	3.5h
研究领域:	Enzyme & Kinase;
Uniprot ID:	P27815
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDE4A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDE4A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDE4A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDE4A in the samples is then determined by comparing the OD of the samples to the standard curve.