鸡血红素加氧酶-1(HMOX1)酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子、生化试剂等全球供应商

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ELK0867

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产品名称:	Chicken HMOX1 (Heme oxygenase 1) ELISA Kit
反应性:	Chicken
别名:	HO1; Hsp32; HMOX1D
反应类型:	Sandwich
灵敏度:	0.32 ng/mL
标准品:	50 ng/mL
检测范围:	0.79-50 ng/mL
样本类型:	tissue homogenates, cell lysates and other biological fluids
反应时长:	3.5h
研究领域:	Enzyme & Kinase;Metabolic pathway;Cardiovascular biology;
Uniprot ID:	P14791
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken HMOX1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken HMOX1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken HMOX1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken HMOX1 in the samples is then determined by comparing the OD of the samples to the standard curve.