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一步法人白介素8(IL8)酶联免疫吸附检测试剂盒
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ELK085ES
规格: 价格:
64T ¥1680.00
112T ¥2400.00
112T*5 ¥10200.00

Overview

产品名称: EasyStep Human IL8(Interleukin 8) ELISA Kit
反应性: Human
别名: IL-8; CXCL8; AMCF-I; GCP1; K60; LECT; LUCT; LYNAP; MDNCF; MONAP; NAF; NAP1; SCYB8; TSG1; B-ENAP; Neutrophil-Activating Protein 1; Granulocyte Chemotactic Protein 1
反应类型: Sandwich
灵敏度: 3.44 pg/mL
标准品: 400 pg/mL
检测范围: 6.25-400 pg/mL
样本类型: Cell culture supernatants
反应时长: 1.5h
研究领域: Cytokine;Infection immunity;
检测原理: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL8, and the Human IL8 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL8. After TMB substrate solution is added, only those wells that contain Human IL8 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL8 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
400.00 1.951 1.856
200.00 1.522 1.427
100.00 1.088 0.993
50.00 0.805 0.710
25.00 0.506 0.411
12.50 0.317 0.222
6.25 0.176 0.081
0.00 0.095 0.000

精密度

板内精密度(测定内的精密度):CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次,以评估分析板内精密度。

板间精密度(测定板间间精密度):CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次,以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的IL8, 做回收率实验,得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 95-105% 100%
EDTA 血浆(n=5) 80-95% 87%
Heparin 血浆(n=5) 92-105% 98%

线性

将添加有IL8的样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 87-96% 85-97% 85-94% 87-96%
EDTA 血浆(n=5) 88-97% 91-98% 86-103% 79-91%
Heparin 血浆(n=5) 85-104% 87-106% 83-92% 82-90%

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