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人磷酸化AKT(S473)酶联免疫吸附检测试剂盒
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ELK0792
规格: 价格:
48T ¥1820.00
96T ¥2600.00
96T*5 ¥11050.00

Overview

产品名称: Human Phospho AKT S473 (p-AKT) ELISA Kit
反应性: Human
别名: p-AKT
反应类型: Sandwich
灵敏度: 52.5 pg/mL
标准品: 10000 pg/mL
检测范围: 156.25-10000 pg/mL
样本类型: tissue homogenates, cell lysates and other biological fluids
反应时长: 3.5h
研究领域: Enzyme & Kinase;
检测原理: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human p-AKT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human p-AKT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human p-AKT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human p-AKT in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
10000.00 2.154 2.059
5000.00 1.638 1.543
2500.00 1.175 1.080
1250.00 0.871 0.776
625.00 0.577 0.482
312.50 0.315 0.220
156.25 0.258 0.163
0.00 0.095 0.000

精密度

板内精密度(测定内的精密度):CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次,以评估分析板内精密度。

板间精密度(测定板间间精密度):CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次,以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的p-AKT, 做回收率实验,得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 90-107% 98%
EDTA 血浆(n=5) 82-98% 90%
Heparin 血浆(n=5) 85-99% 92%

线性

将添加有p-AKT的样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 85-99% 79-93% 81-96% 85-96%
EDTA 血浆(n=5) 87-105% 83-92% 82-96% 88-101%
Heparin 血浆(n=5) 95-106% 85-104% 90-98% 89-105%
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