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小鼠磷酸化AKT(S473)酶联免疫吸附检测试剂盒
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ELK0791
规格: 价格:
48T ¥1820.00
96T ¥2600.00
96T*5 ¥11050.00

Overview

产品名称: Mouse Phospho AKT S473 (p-AKT) ELISA Kit
反应性: Mouse
别名: p-AKT
反应类型: Sandwich
灵敏度: 49.6 pg/mL
标准品: 10000 pg/mL
检测范围: 156.25-10000 pg/mL
样本类型: tissue homogenates, cell lysates and other biological fluids
反应时长: 3.5h
研究领域: Enzyme & Kinase;
检测原理: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse p-AKT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse p-AKT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse p-AKT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse p-AKT in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
10000.00 2.266 2.170
5000.00 1.587 1.491
2500.00 1.282 1.186
1250.00 0.806 0.710
625.00 0.559 0.463
312.50 0.357 0.261
156.25 0.236 0.140
0.00 0.096 0.000

精密度

板内精密度(测定内的精密度):CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次,以评估分析板内精密度。

板间精密度(测定板间间精密度):CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次,以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的p-AKT, 做回收率实验,得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 78-95% 86%
EDTA 血浆(n=5) 89-105% 97%
Heparin 血浆(n=5) 86-99% 92%

线性

将添加有p-AKT的样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 91-102% 79-91% 89-103% 82-95%
EDTA 血浆(n=5) 79-92% 87-103% 79-96% 92-105%
Heparin 血浆(n=5) 85-92% 96-103% 87-98% 95-106%
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