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大鼠牛磺胆酸(TCA)酶联免疫吸附检测试剂盒
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ELK0786
规格： 价格：
48T ￥1960.00
96T ￥2800.00
96T*5 ￥11900.00
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Overview

产品名称: Rat TCA(Taurocholic Acid) ELISA Kit
反应性: Rat
别名: Cholaic Acid; Cholyltaurine; Acidum Cholatauricum
反应类型: Competitive Inhibition
灵敏度: 1.4 ng/mL
标准品: 300 ng/mL
检测范围: 4.69-300 ng/mL
样本类型: Serum, plasma and other biological fluids
反应时长: 2h
研究领域: Signal transduction;
检测原理: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat TCA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TCA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TCA in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL) OD Corrected OD
300.00 0.191
150.00 0.356
75.00 0.524
37.50 0.897
18.75 1.152
9.38 1.522
4.69 1.789
0.00 2.213

精密度

板内精密度（测定内的精密度）：CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次，以评估分析板内精密度。

板间精密度（测定板间间精密度）：CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次，以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的TCA， 做回收率实验，得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 81-103% 87%
EDTA 血浆(n=5) 93-105% 99%
Heparin 血浆(n=5) 84-93% 89%

线性

将添加有TCA的样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 85-96% 87-101% 89-102% 85-98%
EDTA 血浆(n=5) 86-101% 86-101% 87-96% 92-105%
Heparin 血浆(n=5) 83-97% 82-96% 86-98% 93-101%
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