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小鼠磷酸化胰岛素受体β亚单位IR (Phospho) [pY1162/1163]酶联免疫吸附检测试剂盒
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ELK0766
规格： 价格：
48T ￥1960.00
96T ￥2800.00
96T*5 ￥11900.00
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产品名称: Mouse phospho-Insulin Receptor, β Subunit(pTyr1162/1163) ELISA Kit
反应性: Mouse
别名: p-IR
反应类型: Sandwich
灵敏度: 0.68 U/mL
标准品: 100 U/mL
检测范围: 1.57-100 U/mL
样本类型: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
反应时长: 3.5h
研究领域: Signal transduction;Endocrinology;Autoimmunity;
检测原理: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IR (Phospho) [pY1162/1163]. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IR (Phospho) [pY1162/1163]. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IR (Phospho) [pY1162/1163], biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IR (Phospho) [pY1162/1163] in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (U/mL) OD Corrected OD
100.00 2.079 1.978
50.00 1.593 1.492
25.00 1.183 1.082
12.50 0.857 0.756
6.25 0.509 0.408
3.13 0.325 0.224
1.57 0.199 0.098
0.00 0.101 0.000

精密度

板内精密度（测定内的精密度）：CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次，以评估分析板内精密度。

板间精密度（测定板间间精密度）：CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次，以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的IR (Phospho) [pY1162/1163]， 做回收率实验，得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 82-98% 90%
EDTA 血浆(n=5) 82-94% 88%
Heparin 血浆(n=5) 80-97% 88%

线性

将添加有IR (Phospho) [pY1162/1163]的样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 83-97% 84-99% 95-102% 88-103%
EDTA 血浆(n=5) 95-104% 82-96% 92-103% 90-99%
Heparin 血浆(n=5) 89-97% 85-94% 79-96% 85-99%
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