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人抗组织谷氨酰胺转移酶抗体IgA(Anti-tTG-IgA)酶联免疫吸附检测试剂盒
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ELK0752
规格： 价格：
48T ￥1960.00
96T ￥2800.00
96T*5 ￥11900.00
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Overview 文献(1)

产品名称: Human Anti-tTG-IgA (Anti-Tissue Transglutaminase IgA) ELISA Kit
反应性: Human
别名: Anti-Tissue Transglutaminase IgA
反应类型: Sandwich
灵敏度: 1.45 IU/mL
标准品: 300 IU/mL
检测范围: 4.69-300 IU/mL
样本类型: Serum, plasma, tissue homogenates and other biological fluids.
反应时长: 3.5h
研究领域: Enzyme & Kinase;Endocrinology;Neuro science;Autoimmunity;
检测原理: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-tTG-IgA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-tTG-IgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-tTG-IgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-tTG-IgA in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (IU/mL) OD Corrected OD
300.00 2.075 1.988
150.00 1.472 1.385
75.00 1.069 0.982
37.50 0.728 0.641
18.75 0.535 0.448
9.38 0.303 0.216
4.69 0.191 0.104
0.00 0.087 0.000

精密度

板内精密度（测定内的精密度）：CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次，以评估分析板内精密度。

板间精密度（测定板间间精密度）：CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次，以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的Anti-tTG-IgA， 做回收率实验，得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 85-97% 91%
EDTA 血浆(n=5) 82-97% 89%
Heparin 血浆(n=5) 87-95% 91%

线性

将添加有Anti-tTG-IgA的样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 95-102% 83-96% 97-105% 85-97%
EDTA 血浆(n=5) 78-91% 86-92% 89-96% 91-97%
Heparin 血浆(n=5) 81-93% 78-91% 87-98% 95-107%
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