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小鼠消退素E1(RvE1)酶联免疫吸附检测试剂盒
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ELK0495
规格： 价格：
48T ￥1960.00
96T ￥2800.00
96T*5 ￥11900.00
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产品名称: Mouse RvE1(Resolvin E1) ELISA Kit
反应性: Mouse
别名: RvE1
反应类型: Competitive Inhibition
灵敏度: 6.97 pg/mL
标准品: 1000 pg/mL
检测范围: 15.63-1000 pg/mL
样本类型: serum, plasma, cell lysates, cell culture supernates and other biological fluids
反应时长: 2.5h
研究领域: Immunology & Inflammation
检测原理: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse RvE1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse RvE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse RvE1 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
1000.00 0.221
500.00 0.375
250.00 0.557
125.00 0.899
62.50 1.119
31.25 1.587
15.63 1.887
0.00 2.316

精密度

板内精密度（测定内的精密度）：CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次，以评估分析板内精密度。

板间精密度（测定板间间精密度）：CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次，以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的RvE1， 做回收率实验，得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 91-105% 98%
EDTA 血浆(n=5) 95-107% 101%
Heparin 血浆(n=5) 85-97% 91%

线性

将添加有RvE1的样本分别稀释2倍，4倍，8倍，16倍做回收实验，得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 83-101% 90-99% 87-103% 89-97%
EDTA 血浆(n=5) 85-94% 79-92% 87-101% 82-90%
Heparin 血浆(n=5) 85-99% 82-96% 92-101% 82-90%
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