产品中心

大鼠发动蛋白1样蛋白(DNM1L)酶联免疫吸附检测试剂盒
一键复制产品信息
ELK0215
规格: 价格:
48T ¥1820.00
96T ¥2600.00
96T*5 ¥11050.00

Overview

产品名称: Rat DNM1L(Dynamin 1 Like Protein) ELISA Kit
反应性: Rat
别名: DRP1; DLP1; DVLP; DYMPLE; HDYNIV; VPS1; Dynamin-related protein 1; Dnm1p/Vps1p-like protein; Dynamin family member proline-rich carboxyl-terminal domain less
反应类型: Sandwich
灵敏度: 0.064 ng/mL
标准品: 5000 pg/mL
检测范围: 78.13-5000 pg/mL
样本类型: serum, plasma and other biological fluids
反应时长: 3.5h
研究领域: Infection immunity;
Uniprot ID: O35303
检测原理: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat DNM1L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat DNM1L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat DNM1L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat DNM1L in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
5000.00 2.157 2.076
2500.00 1.657 1.576
1250.00 1.174 1.093
625.00 0.873 0.792
312.50 0.528 0.447
156.25 0.423 0.342
78.13 0.248 0.167
0.00 0.081 0.000

精密度

板内精密度(测定内的精密度):CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次,以评估分析板内精密度。

板间精密度(测定板间间精密度):CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次,以评估分析板间的精密度。

回收率

分别往不同样本中添加已知浓度的DNM1L, 做回收率实验,得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 94-107% 100%
EDTA 血浆(n=5) 94-105% 98%
Heparin 血浆(n=5) 82-95% 88%

线性

将添加有DNM1L的样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 88-102% 86-92% 91-103% 89-97%
EDTA 血浆(n=5) 88-103% 83-94% 92-105% 89-97%
Heparin 血浆(n=5) 85-97% 87-96% 82-95% 92-105%
关闭

在线咨询

Online consultation

  • 在线咨询
  • 技术支持

关注微信公众号

微信扫一扫立即咨询

微信扫一扫立即咨询

关闭