马硫代巴比妥酸反应物(TBARS)酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子、生化试剂等全球供应商

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ELK0022

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产品名称:	Horse TBARS(Thiobarbituric Acid Reactive Substance) ELISA Kit
反应性:	Horse
别名:	TBARS; Thiobarbituric Acid Reactive Substance
反应类型:	Competitive Inhibition
灵敏度:	3.55 nmol/mL
标准品:	400 nmol/mL
检测范围:	6.25-400 nmol/mL
样本类型:	Serum, plasma, cell lysates, cell culture supernates and other biological fluids
反应时长:	2h
研究领域:	Metabolic pathway;Hepatology;Hormone metabolism;
检测原理:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse TBARS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse TBARS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse TBARS in the samples is then determined by comparing the OD of the samples to the standard curve.