山羊干扰素α(IFNα)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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山羊干扰素α(IFNα)酶联免疫吸附检测试剂盒

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ELK0017

规格：	价格：
48T	￥2240.00
96T	￥3200.00
96T*5	￥13600.00

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Overview

Product name:	Goat IFNα(Interferon Alpha) ELISA Kit
Reactivity:	Goat
Alternative Names:	IFNα1; IFL; LeIF D; IFN; IFN-Alpha; IFNα13; IFN-A; IFNαP22; IFN-Alpha 1b; Interferon Alpha 1b; Interferon, Leukocytic; IFN, Leukocyte; Interferon alpha-D
Assay Type:	Sandwich
Sensitivity:	5.5 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample Type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length:	3.5h
Research Area:	Cytokine;Infection immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Goat IFNα. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Goat IFNα. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Goat IFNα, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Goat IFNα in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.157	2.061
500.00	1.627	1.531
250.00	1.284	1.188
125.00	0.838	0.742
62.50	0.509	0.413
31.25	0.313	0.217
15.63	0.227	0.131
0.00	0.096	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IFNα and the recovery rates were calculated by comparing the measured value to the expected amount of IFNα in samples.

Matrix	Recovery range	Average
serum(n=5)	85-97%	91%
EDTA plasma(n=5)	96-107%	101%
Heparin plasma(n=5)	90-105%	97%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFNα and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.