Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human ARFGEF2. AA range:1491-1540
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
210kD
GeneID:
10564
Human Swiss-Prot No:
Q9Y6D5
Cellular localization:
Cytoplasm. Membrane. Golgi apparatus. Cytoplasm, perinuclear region. Golgi apparatus, trans-Golgi network . Endosome . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell projection, dendrite . Cytoplasmic vesicle . Cell junction, synapse . Cytoplasm, cytoskeleton . Translocates from cytoplasm to membranes upon cAMP treatment. Localized in recycling endosomes.
Background:
ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. The protein encoded by this gene is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. It contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition. [provided by RefSeq, Jul 2008],