eIF4A1 Mouse mAb-科鹿（武汉）生物科技有限责任公司

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eIF4A1 Mouse mAb

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EM1136

规格：	价格：
50µL	￥1280.00
100µL	￥1980.00

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Overview

Product name:	eIF4A1 Mouse mAb
Reactivity:	Human, Rat, Mouse
Alternative Names:	DDX2, eIF 4A I, Eukaryotic initiation factor 4A-1, ATP-dependent RNA helicase eIF4A-1,DDX2A
Source:	Mouse
Dilutions:	WB 1:1,000-3,000 IF 1:100-200 IHC 1:100-200
Immunogen:	Synthetic Peptide
Storage:	PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Clonality:	Monoclonal
Isotype:	IgG1
Concentration:	1mg/mL
Observed Band:	48kDa
GeneID:	1973
Human Swiss-Prot No:	P60842
Cellular localization:	Cytosol,Extracellular region or secreted
Background:	The eukaryotic initiation factor-4A (eIF4A) family consists of 3 closely related proteins EIF4A1, EIF4A2, and EIF4A3. These factors are required for the binding of mRNA to 40S ribosomal subunits. Eukaryotic initiation factor (eIF) complex 2 forms a ternary complex with GTP and the initiator Met-tRNA – this process is regulated by guanine nucleotide exchange and phosphorylation and serves as the main regulatory element of the bottleneck of protein expression.

Western blot analysis of 1) 293T ,2) Hela, 3) HepG2, 4) Mouse Brain tissue, 5) Rat Brain tissue with EM1136 diluted at 1:2,000.
IF analysis of Hela with EM1136(Left) and DAPI (Right) diluted at 1:100.
Immunohistochemical analysis of paraffin-embedded Human Lung Caricnoma using eIF4A1（EM1136）Mouse mAb diluted at 1:200.

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