人甘露糖结合蛋白C(MBP-C/MBL2)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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人甘露糖结合蛋白C(MBP-C/MBL2)酶联免疫吸附检测试剂盒

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ELK9102

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview

Product name:	Human MBP-C/MBL2(Mannose Binding Protein C/Mannose Binding Lectin) ELISA Kit
Reactivity:	Human
Alternative Names:	MBL2; COLEC1; HSMBPC; MBP1; MBP; Collectin-1; Mannose-binding protein C; Mannan Binding Protein; Mannose-Binding Lectin(Protein C)2,Soluble(Opsonic Defect); EPCA2; Early Prostate Cancer Antigen 2
Assay Type:	Sandwich
Sensitivity:	2.54 ng/mL
Standard:	400 ng/mL
Detection Range:	6.25-400 ng/mL
Sample type:	Serum, plasma and other biological fluids
Assay length:	3.5h
Research Area:	Signal Transduction; Cancer
Uniprot ID:	P11226
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MBP-C/MBL2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MBP-C/MBL2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MBP-C/MBL2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MBP-C/MBL2 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
400.00	1.932	1.845
200.00	1.515	1.428
100.00	1.067	0.980
50.00	0.892	0.805
25.00	0.553	0.466
12.50	0.389	0.302
6.25	0.161	0.074
0.00	0.087	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant MBP-C/MBL2 and the recovery rates were calculated by comparing the measured value to the expected amount of MBP-C/MBL2 in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA plasma(n=5)	83-95%	89%
Heparin plasma(n=5)	90-105%	97%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MBP-C/MBL2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.