脱氢表雄酮硫酸酯(DHEA-S)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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脱氢表雄酮硫酸酯(DHEA-S)酶联免疫吸附检测试剂盒

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ELK8370

规格：	价格：
48T	￥2240.00
96T	￥3200.00

下载说明 ①

Overview

Product name:	DHEA-S(Dehydroepiandrosterone sulfate) ELISA Kit
Reactivity:	General
Alternative Names:	Androstenolone sulfate; Prasterone sulfate; Androst-5-en-3β-ol-17-one 3β-sulfate
Assay Type:	Competitive Inhibition
Sensitivity:	4.44 ng/mL
Standard:	400 ng/mL
Detection Range:	6.25-400 ng/mL
Sample type:	Serum, plasma and other biological fluids.
Assay length:	2h
Research Area:	Endocrinology;Reproductive science;Hormone metabolism;Rheumatology;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DHEA-S. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DHEA-S. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DHEA-S in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
400.00	0.185
200.00	0.397
100.00	0.611
50.00	0.997
25.00	1.139
12.50	1.578
6.25	1.889
0.00	2.101

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant DHEA-S and the recovery rates were calculated by comparing the measured value to the expected amount of DHEA-S in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	88-103%	95%
Heparin plasma(n=5)	95-107%	96%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DHEA-S and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.