人抗免疫球蛋白A抗体(Anti-IgA)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

产品中心

人抗免疫球蛋白A抗体(Anti-IgA)酶联免疫吸附检测试剂盒

一键复制产品信息

ELK8046

规格：	价格：
48T	￥2240.00
96T	￥3200.00

下载说明 ①

Overview

Product name:	Human Anti-IgA(Anti-Immunoglobulin A Antibody) ELISA Kit
Reactivity:	Human
Alternative Names:	anti-IgA
Assay Type:	Sandwich
Sensitivity:	2.38 ng/mL
Standard:	400 ng/mL
Detection Range:	6.25-400 ng/mL
Sample type:	serum, plasma and other biological fluids
Assay length:	3.5h
Research Area:	Infection immunity;Immune molecule;Hematology;Pulmonology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-IgA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-IgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-IgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-IgA in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
400.00	2.045	1.964
200.00	1.527	1.446
100.00	1.088	1.007
50.00	0.892	0.811
25.00	0.547	0.466
12.50	0.403	0.322
6.25	0.179	0.098
0.00	0.081	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Anti-IgA and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-IgA in samples.

Matrix	Recovery range	Average
serum(n=5)	83-97%	91%
EDTA plasma(n=5)	82-94%	88%
Heparin plasma(n=5)	94-105%	98%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-IgA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.