人血管紧张素1-9(Ang1-9)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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人血管紧张素1-9(Ang1-9)酶联免疫吸附检测试剂盒

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ELK7609

规格：	价格：
48T	￥2240.00
96T	￥3200.00

下载说明 ①

Overview

Product name:	Human Ang1-9(Angiotensin 1-9) ELISA Kit
Reactivity:	Human
Alternative Names:	Ang1-9
Assay Type:	Sandwich
Sensitivity:	5.31 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length:	3.5h
Research Area:	Endocrinology;Cardiovascular biology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Ang1-9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Ang1-9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Ang1-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Ang1-9 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.274	2.188
500.00	1.621	1.535
250.00	1.217	1.131
125.00	0.937	0.851
62.50	0.611	0.525
31.25	0.336	0.250
15.63	0.236	0.150
0.00	0.086	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant Ang1-9 and the recovery rates were calculated by comparing the measured value to the expected amount of Ang1-9 in samples.

Matrix	Recovery range	Average
serum(n=5)	81-95%	88%
EDTA plasma(n=5)	95-103%	99%
Heparin plasma(n=5)	92-102%	97%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ang1-9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.