人PP2A癌症抑制抑制因子(CIP2A)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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人PP2A癌症抑制抑制因子(CIP2A)酶联免疫吸附检测试剂盒

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ELK7088

规格：	价格：
48T	￥2240.00
96T	￥3200.00

下载说明 ①

Overview

Product name:	Human CIP2A(Cancerous Inhibitor Of PP2A) ELISA Kit
Reactivity:	Human
Alternative Names:	p90; KIAA1524
Assay Type:	Sandwich
Sensitivity:	0.057 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample type:	Serum, plasma and other biological fluids
Assay length:	3.5h
Research Area:	Tumor immunity;
Uniprot ID:	Q8TCG1
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CIP2A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CIP2A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CIP2A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CIP2A in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
10.00	1.846	1.748
5.00	1.636	1.538
2.50	1.198	1.100
1.25	0.752	0.654
0.63	0.508	0.410
0.32	0.318	0.220
0.16	0.206	0.108
0.00	0.098	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CIP2A and the recovery rates were calculated by comparing the measured value to the expected amount of CIP2A in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	93%
EDTA plasma(n=5)	80-95%	87%
Heparin plasma(n=5)	92-106%	100%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CIP2A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.