产品中心

小鼠心肌肌钙蛋白T(cTnT/TNNT2)酶联免疫吸附检测试剂盒
ELK6207
规格: 价格:
48T ¥2240.00
96T ¥3200.00

Overview 文献

Product name: Mouse cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit
Reactivity: Mouse
Alternative Names: CMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T; TNNT2; Troponin T Type 2, Cardiac
Assay Type: Sandwich
Sensitivity: 5.5 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cardiovascular biology;
Uniprot ID: P50752
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse cTnT/TNNT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse cTnT/TNNT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse cTnT/TNNT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse cTnT/TNNT2 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
1000.00 1.952 1.856
500.00 1.586 1.490
250.00 1.147 1.051
125.00 0.895 0.799
62.50 0.547 0.451
31.25 0.354 0.258
15.63 0.142 0.046
0.00 0.096 0.000

精密度

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant cTnT/TNNT2 and the recovery rates were calculated by comparing the measured value to the expected amount of cTnT/TNNT2 in samples.
Matrix Recovery range Average
serum(n=5) 80-95% 87%
EDTA plasma(n=5) 78-92% 85%
Heparin plasma(n=5) 78-96% 87%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of cTnT/TNNT2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 92-104% 82-96% 95-102% 87-98%
EDTA plasma(n=5) 87-98% 92-104% 82-96% 95-102%
Heparin plasma(n=5) 89-97% 85-94% 79-96% 85-99%
关闭

在线咨询

Online consultation

  • 在线咨询
  • 技术支持

关注微信公众号

微信扫一扫立即咨询

微信扫一扫立即咨询

关闭