小鼠脑型肌酸激酶(CKB)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

产品中心

小鼠脑型肌酸激酶(CKB)酶联免疫吸附检测试剂盒

一键复制产品信息

ELK5636

规格：	价格：
48T	￥2240.00
96T	￥3200.00

下载说明 ①

Overview 文献(1)

Product name:	Mouse CKB(Creatine Kinase, Brain) ELISA Kit
Reactivity:	Mouse
Alternative Names:	CK-BB; CKBB; CK-B; CK-1
Assay Type:	Sandwich
Sensitivity:	0.127 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample type:	Tissue homogenates, cell lysates and other biological fluids.
Assay length:	3.5h
Research Area:	Enzyme & Kinase;Metabolic pathway;Cardiovascular biology;Neuro science;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CKB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CKB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CKB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CKB in the samples is then determined by comparing the OD of the samples to the standard curve.

Biomaterials Advances

Mechanism of selenium-doped black phosphorus nanosheets wrapped with biomimetic tumor cell membrane for prostate cancer immunotherapy

日期：2025-06-16 IF：5.5

标准曲线

Concentration (ng/mL)	OD	Corrected OD
20.00	1.954	1.860
10.00	1.582	1.488
5.00	1.167	1.073
2.50	0.895	0.801
1.25	0.518	0.424
0.63	0.387	0.293
0.32	0.226	0.132
0.00	0.094	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CKB and the recovery rates were calculated by comparing the measured value to the expected amount of CKB in samples.

Matrix	Recovery range	Average
serum(n=5)	88-103%	95%
EDTA plasma(n=5)	95-107%	101%
Heparin plasma(n=5)	80-95%	87%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CKB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.