规格：
价格：
下载说明 ①Overview 文献(1)
日期：2025-06-16
IF：4.7标准曲线
| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 5000.00 | 2.103 | 2.026 |
| 2500.00 | 1.608 | 1.531 |
| 1250.00 | 1.233 | 1.156 |
| 625.00 | 0.937 | 0.860 |
| 312.50 | 0.527 | 0.450 |
| 156.25 | 0.361 | 0.284 |
| 78.13 | 0.206 | 0.129 |
| 0.00 | 0.077 | 0.000 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant NT-ProBNP and the recovery rates were calculated by comparing the measured value to the expected amount of NT-ProBNP in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 86-99% | 92% |
| EDTA plasma(n=5) | 80-95% | 87% |
| Heparin plasma(n=5) | 90-105% | 97% |
线性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-ProBNP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 85-100% | 84-98% | 95-101% | 88-102% |
| EDTA plasma(n=5) | 88-104% | 84-98% | 95-105% | 88-102% |
| Heparin plasma(n=5) | 87-96% | 91-101% | 87-98% | 89-103% |
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