人高迁移率族AT Hook蛋白2(HMGA2)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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人高迁移率族AT Hook蛋白2(HMGA2)酶联免疫吸附检测试剂盒

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ELK3464

规格：	价格：
48T	￥1960.00
96T	￥2800.00

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Overview 文献(1)

Product name:	Human HMGA2(High Mobility Group AT Hook Protein 2) ELISA Kit
Reactivity:	Human
Alternative Names:	BABL; HMGI-C; HMGIC; LIPO; High mobility group protein HMGI-C
Assay Type:	Sandwich
Sensitivity:	0.128 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample type:	tissue homogenates, cell lysates and other biological fluids
Assay length:	3.5h
Research Area:	Metabolic pathway;Tumor immunity;Endocrinology;Nutrition metabolism;
Uniprot ID:	P52926
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HMGA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HMGA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HMGA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HMGA2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Journal of Cancer Research and Clinical Oncology

Investigating the role of HMGA2 plasma level as a diagnostic marker in bladder urothelial carcinoma patients

日期：2025-05-13 IF：2.7

标准曲线

Concentration (ng/mL)	OD	Corrected OD
20.00	1.954	1.866
10.00	1.608	1.520
5.00	1.175	1.087
2.50	0.897	0.809
1.25	0.563	0.475
0.63	0.239	0.151
0.32	0.208	0.120
0.00	0.088	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant HMGA2 and the recovery rates were calculated by comparing the measured value to the expected amount of HMGA2 in samples.

Matrix	Recovery range	Average
serum(n=5)	85-99%	92%
EDTA plasma(n=5)	78-92%	85%
Heparin plasma(n=5)	78-90%	84%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HMGA2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.