人组织因子通道抑制因子2(TFPI2)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

产品中心

人组织因子通道抑制因子2(TFPI2)酶联免疫吸附检测试剂盒

一键复制产品信息

ELK2691

规格：	价格：
48T	￥1960.00
96T	￥2800.00

下载说明 ①

Overview

Product name:	Human TFPI2(Tissue Factor Pathway Inhibitor 2) ELISA Kit
Reactivity:	Human
Alternative Names:	PP5; Placental protein 5
Assay Type:	Sandwich
Sensitivity:	12.2 pg/mL
Standard:	2000 pg/mL
Detection Range:	31.25-2000 pg/mL
Sample type:	Plasma.
Assay length:	3.5h
Research Area:	Hematology;Cardiovascular biology;
Uniprot ID:	P48307
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TFPI2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TFPI2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TFPI2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TFPI2 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
2000.00	1.989	1.902
1000.00	1.628	1.541
500.00	1.043	0.956
250.00	0.872	0.785
125.00	0.589	0.502
62.50	0.326	0.239
31.25	0.190	0.103
0.00	0.087	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant TFPI2 and the recovery rates were calculated by comparing the measured value to the expected amount of TFPI2 in samples.

Matrix	Recovery range	Average
serum(n=5)	95-107%	101%
EDTA plasma(n=5)	82-95%	88%
Heparin plasma(n=5)	88-102%	95%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TFPI2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.