人爱帕琳肽12(AP12)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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人爱帕琳肽12(AP12)酶联免疫吸附检测试剂盒

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ELK1635

规格：	价格：
48T	￥1960.00
96T	￥2800.00

下载说明 ①

Overview

Product name:	Human AP12(Apelin 12) ELISA Kit
Reactivity:	Human
Alternative Names:	APLN12
Assay Type:	Competitive Inhibition
Sensitivity:	25.28 pg/mL
Standard:	6000 pg/mL
Detection Range:	93.75-6000 pg/mL
Sample type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length:	2h
Research Area:	Metabolic pathway;Endocrinology;Cardiovascular biology;Gastroenterology;Hormone metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP12 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
6000.00	0.235
3000.00	0.433
1500.00	0.711
750.00	0.967
375.00	1.257
187.50	1.565
93.75	1.877
0.00	2.197

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant AP12 and the recovery rates were calculated by comparing the measured value to the expected amount of AP12 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-95%	87%
EDTA plasma(n=5)	85-98%	91%
Heparin plasma(n=5)	96-107%	102%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AP12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.