小鼠载脂蛋白H(APOH)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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小鼠载脂蛋白H(APOH)酶联免疫吸附检测试剂盒

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ELK11131

规格：	价格：
48T	￥1960.00
96T	￥2800.00

下载说明 ①

Overview

Product name:	Mouse APOH(Apolipoprotein H) ELISA Kit
Reactivity:	Mouse
Alternative Names:	Apo-H; B2G1; BG; B2-GP1; B2GP1; Previously; Beta 2 Glycoprotein 1; APC inhibitor; Activated protein C-binding protein; Anticardiolipin cofactor; Beta(2)GPI
Assay Type:	Sandwich
Sensitivity:	1.8 µg/mL
Standard:	500 µg/mL
Detection Range:	7.82-500 µg/mL
Sample type:	Serum, plasma, saliva and other biological fluids.
Assay length:	3.5h
Research Area:	Metabolic pathway;Cardiovascular biology;
Uniprot ID:	Q01339
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse APOH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse APOH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse APOH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse APOH in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (µg/mL)	OD	Corrected OD
500.00	2.201	2.115
250.00	1.782	1.696
125.00	1.119	1.033
62.50	0.752	0.666
31.25	0.587	0.501
15.63	0.324	0.238
7.82	0.161	0.075
0.00	0.086	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant APOH and the recovery rates were calculated by comparing the measured value to the expected amount of APOH in samples.

Matrix	Recovery range	Average
serum(n=5)	82-95%	88%
EDTA plasma(n=5)	95-107%	101%
Heparin plasma(n=5)	87-99%	93%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of APOH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.