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标准曲线
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 20.00 | 2.201 | 2.076 |
| 10.00 | 1.717 | 1.592 |
| 5.00 | 1.251 | 1.126 |
| 2.50 | 0.806 | 0.681 |
| 1.25 | 0.539 | 0.414 |
| 0.63 | 0.364 | 0.239 |
| 0.32 | 0.258 | 0.133 |
| 0.00 | 0.125 | 0.000 |
精密度
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
回收率
Matrices listed below were spiked with certain level of recombinant E-Cad and the recovery rates were calculated by comparing the measured value to the expected amount of E-Cad in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 85-97% | 91% |
| EDTA plasma(n=5) | 95-107% | 101% |
| Heparin plasma(n=5) | 82-95% | 88% |
线性
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of E-Cad and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 95-102% | 96-104% | 87-98% | 95-107% |
| EDTA plasma(n=5) | 87-97% | 86-92% | 89-103% | 89-98% |
| Heparin plasma(n=5) | 79-85% | 87-96% | 82-95% | 89-103% |
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