大鼠8-羟基脱氧鸟苷(8-OHdG)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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大鼠8-羟基脱氧鸟苷(8-OHdG)酶联免疫吸附检测试剂盒

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ELK10967

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview 文献(2)

Product name:	Rat 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit
Reactivity:	Rat
Alternative Names:	8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG; 7,8-dihydrodeoxyguanosine
Assay Type:	Competitive Inhibition
Sensitivity:	0.46 ng/mL
Standard:	100 ng/mL
Detection Range:	1.56-100 ng/mL
Sample type:	serum, plasma and other biological fluids
Assay length:	2h
Research Area:	Metabolic pathway;Tumor immunity;Infection immunity;Endocrinology;Hematology;Hepatology;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat 8-OHdG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat 8-OHdG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve.

BIOLOGICAL TRACE ELEMENT RESEARCH

Molecular Changes Associated with Inflammation and Reproduction in Cadmium-Induced Testicular Toxicity: Mitigating Effect of Lactobacillus plantarum

日期：2025-05-13 IF：3.4
Naunyn-Schmiedeberg's Archives of Pharmacology

Cilostazol alleviates imatinib-induced myocardial injury in rats by modulating the TGF-β1/MAPK, SHC/Grb2/SOS signaling pathways and upregulating miRNA-195-5P

日期：2025-04-17 IF：3.1

标准曲线

Concentration (ng/mL)	OD	Corrected OD
100.00	0.189
50.00	0.332
25.00	0.621
12.50	0.897
6.25	1.253
3.13	1.583
1.57	1.761
0.00	2.211

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant 8-OHdG and the recovery rates were calculated by comparing the measured value to the expected amount of 8-OHdG in samples.

Matrix	Recovery range	Average
serum(n=5)	78-92%	85%
EDTA plasma(n=5)	87-99%	93%
Heparin plasma(n=5)	80-95%	97%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-OHdG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.