鸡免疫球蛋白A(IgA)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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鸡免疫球蛋白A(IgA)酶联免疫吸附检测试剂盒

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ELK10825

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview 文献(1)

Product name:	Chicken IgA(Immunoglobulin A) ELISA Kit
Reactivity:	Chicken
Alternative Names:	Immunoglobulin A
Assay Type:	Sandwich
Sensitivity:	0.97 µg/mL
Standard:	200 µg/mL
Detection Range:	3.13-200 µg/mL
Sample type:	serum, plasma and other biological fluids
Assay length:	3.5h
Research Area:	Infection immunity;Immune molecule;Hematology;Pulmonology;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chicken IgA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken IgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chicken IgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken IgA in the samples is then determined by comparing the OD of the samples to the standard curve.

POULTRY SCIENCE

Polysaccharides of Atractylodes macrocephala Koidz alleviate bursa of Fabricius involution in Magang geese by inhibiting the Wnt signaling pathway

日期：2025-06-16 IF：3.8

标准曲线

Concentration (µg/mL)	OD	Corrected OD
200.00	2.045	1.972
100.00	1.536	1.463
50.00	1.123	1.050
25.00	0.886	0.813
12.50	0.518	0.445
6.25	0.308	0.235
3.13	0.221	0.148
0.00	0.073	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IgA and the recovery rates were calculated by comparing the measured value to the expected amount of IgA in samples.

Matrix	Recovery range	Average
serum(n=5)	80-96%	88%
EDTA plasma(n=5)	87-99%	93%
Heparin plasma(n=5)	83-99%	95%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IgA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.