人抗21-羟化酶抗体(21-OH Ab)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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人抗21-羟化酶抗体(21-OH Ab)酶联免疫吸附检测试剂盒

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ELK10071

规格：	价格：
48T	￥1960.00
96T	￥2800.00

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Overview

Product name:	Human anti-21-OH Ab(anti 21-hydroxylase antibody) ELISA Kit
Reactivity:	Human
Alternative Names:	21-OH Ab
Assay Type:	Sandwich
Sensitivity:	0.189 ng/mL
Standard:	20 ng/mL
Detection Range:	0.32-20 ng/mL
Sample type:	serum, plasma,Tissue homogenates and other biological fluids.
Assay length:	3.5h
Research Area:	Immunology
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 21-OH Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 21-OH Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 21-OH Ab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 21-OH Ab in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL)	OD	Corrected OD
20.00	2.258	2.165
10.00	1.587	1.494
5.00	1.136	1.043
2.50	0.757	0.664
1.25	0.496	0.403
0.63	0.326	0.233
0.32	0.152	0.059
0.00	0.093	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant 21-OH Ab and the recovery rates were calculated by comparing the measured value to the expected amount of 21-OH Ab in samples.

Matrix	Recovery range	Average
serum(n=5)	96-108%	102%
EDTA plasma(n=5)	87-102%	94%
Heparin plasma(n=5)	82-95%	88%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 21-OH Ab and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.