人细胞角蛋白18-M30(CK 18-M30)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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人细胞角蛋白18-M30(CK 18-M30)酶联免疫吸附检测试剂盒

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ELK0871

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview

Product name:	Human CK 18-M30 (Cytokeratin 18-M30) ELISA Kit
Reactivity:	Human
Alternative Names:	K18-M30; CK-18-M30; CYK18-M30
Assay Type:	Sandwich
Sensitivity:	7 µIU/mL
Standard:	1000 µIU/mL
Detection Range:	15.63-1000 µIU/mL
Sample type:	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length:	3.5h
Research Area:	Cancer of the liver; Viral hepatitis
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CK 18-M30. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CK 18-M30. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CK 18-M30, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CK 18-M30 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (µIU/mL)	OD	Corrected OD
1000.00	1.972	1.883
500.00	1.507	1.418
250.00	1.107	1.018
125.00	0.671	0.582
62.50	0.479	0.390
31.25	0.312	0.223
15.63	0.196	0.107
0.00	0.089	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant CK 18-M30 and the recovery rates were calculated by comparing the measured value to the expected amount of CK 18-M30 in samples.

Matrix	Recovery range	Average
serum(n=5)	85-95%	90%
EDTA plasma(n=5)	88-103%	95%
Heparin plasma(n=5)	83-95%	89%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CK 18-M30 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.