人2,3-二磷酸甘油酸(2,3-DPG)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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人2,3-二磷酸甘油酸(2,3-DPG)酶联免疫吸附检测试剂盒

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ELK0778

规格：	价格：
48T	￥2240.00
96T	￥3200.00

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Overview

Product name:	Human 2,3-DPG(2,3-Diphosphoglycerate) ELISA Kit
Reactivity:	Human
Alternative Names:	2,3DPG
Assay Type:	Sandwich
Sensitivity:	0.14 µmol/mL
Standard:	20 µmol/mL
Detection Range:	0.32-20 µmol/mL
Sample type:	Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Assay length:	3.5h
Research Area:	Metabolic pathway
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human 2,3-DPG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human 2,3-DPG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human 2,3-DPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human 2,3-DPG in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (µmol/mL)	OD	Corrected OD
20.00	2.154	2.059
10.00	1.587	1.492
5.00	1.184	1.089
2.50	0.866	0.771
1.25	0.519	0.424
0.63	0.336	0.241
0.32	0.190	0.095
0.00	0.095	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant 2,3-DPG and the recovery rates were calculated by comparing the measured value to the expected amount of 2,3-DPG in samples.

Matrix	Recovery range	Average
serum(n=5)	90-105%	97%
EDTA plasma(n=5)	80-92%	86%
Heparin plasma(n=5)	88-102%	95%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 2,3-DPG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.