¥2240.00/64T ¥3200.00/112T
64T | ¥2240.00 |
112T | ¥3200.00 |
Product name: | EasyStep Human vWF(Von Willebrand Factor) ELISA Kit |
Reactivity: | Human |
Alternative Names: | F8VWF; VWD; von Willebrand antigen 2 |
Assay Type: | Sandwich |
Sensitivity: | 3.97 ng/mL |
Standard: | 1000 ng/mL |
Detection Range: | 15.63-1000 ng/mL |
Sample type: | Serum, plasma |
Assay length: | 1.5h |
Research Area: | Signal transduction;Hematology; |
Test principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human vWF, and the Human vWF standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human vWF. After TMB substrate solution is added, only those wells that contain Human vWF and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human vWF in the samples is then determined by comparing the OD of the samples to the standard curve. |
Concentration (ng/mL) | OD | Corrected OD |
---|---|---|
1000.00 | 2.167 | 2.073 |
500.00 | 1.572 | 1.478 |
250.00 | 1.035 | 0.941 |
125.00 | 0.933 | 0.839 |
62.50 | 0.632 | 0.538 |
31.25 | 0.325 | 0.231 |
15.63 | 0.152 | 0.058 |
0.00 | 0.094 | 0.000 |
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Matrix | Recovery range | Average |
---|---|---|
serum(n=5) | 85-95% | 90% |
EDTA plasma(n=5) | 83-97% | 90% |
Heparin plasma(n=5) | 81-95% | 88% |
Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
---|---|---|---|---|
serum(n=5) | 88-99% | 81-93% | 89-97% | 85-94% |
EDTA plasma(n=5) | 81-93% | 86-98% | 91-103% | 82-95% |
Heparin plasma(n=5) | 87-95% | 85-98% | 82-97% | 92-101% |
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