人激活素A(ACV-A)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

产品中心

人激活素A(ACV-A)酶联免疫吸附检测试剂盒

一键复制产品信息

ELK0174

规格：	价格：
48T	￥1960.00
96T	￥2800.00

下载说明 ①

Overview

Product name:	Human ACV-A(Activin A) ELISA Kit
Reactivity:	Human
Alternative Names:	ACVA; Activin Beta A Beta A Homodimer
Assay Type:	Sandwich
Sensitivity:	4.71 pg/mL
Standard:	1000 pg/mL
Detection Range:	15.63-1000 pg/mL
Sample type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length:	3.5h
Research Area:	Endocrinology;Reproductive science;Hormone metabolism;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ACV-A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ACV-A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ACV-A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ACV-A in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.274	2.169
500.00	1.527	1.422
250.00	1.034	0.929
125.00	0.841	0.736
62.50	0.521	0.416
31.25	0.364	0.259
15.63	0.146	0.041
0.00	0.105	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant ACV-A and the recovery rates were calculated by comparing the measured value to the expected amount of ACV-A in samples.

Matrix	Recovery range	Average
serum(n=5)	92-107%	99%
EDTA plasma(n=5)	87-99%	93%
Heparin plasma(n=5)	78-92%	85%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACV-A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.