一步法人前列腺特异性抗原(PSA)酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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ELK011ES

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产品名称:	EasyStep Human PSA(ProstateSpecific Antigen) ELISA Kit
反应性:	Human
别名:	KLK3; APS; KLK2A1; hK3; Kallikrein-3; Kallikrein-Related Peptidase 3; Gamma-seminoprotein
反应类型:	Sandwich
灵敏度:	80 pg/mL
标准品:	20 ng/mL
检测范围:	0.32-20 ng/mL
样本类型:	Serum, plasma
反应时长:	1.5h
研究领域:	Tags & Cell Markers;Neuroscience;Signal Transduction;Cancer
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSA, and the Human PSA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human PSA. After TMB substrate solution is added, only those wells that contain Human PSA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PSA in the samples is then determined by comparing the OD of the samples to the standard curve.