一步法人脑钠素(BNP)酶联免疫吸附检测试剂盒-ELK科鹿生物-ELISA试剂盒、抗体、蛋白、分子

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ELK007ES

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产品名称:	EasyStep Human BNP(Brain Natriuretic Peptide) ELISA Kit
反应性:	Human
别名:	GC-B; B-Type Natriuretic Peptide; Ventricular Natriuretic Peptide; Gamma-brain natriuretic peptide; Brain natriuretic peptide 32
反应类型:	Sandwich
灵敏度:	6.8 pg/mL
标准品:	2000 pg/mL
检测范围:	31.25-2000 pg/mL
样本类型:	Serum, plasma
反应时长:	1.5h
研究领域:	Cardiovascular biology;Neuro science;Hormone metabolism;
检测原理:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BNP, and the Human BNP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human BNP. After TMB substrate solution is added, only those wells that contain Human BNP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BNP in the samples is then determined by comparing the OD of the samples to the standard curve.