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一步法人胱抑素C(Cys-C)酶联免疫吸附检测试剂盒
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ELK005ES
规格: 价格:
64T ¥1960.00
112T ¥2800.00
112T*5 ¥11900.00

Overview

产品名称: EasyStep Human Cys-C(CystatinC) ELISA Kit
反应性: Human
别名: CST3; Cystatin 3; ARMD11; Post-Gamma-Globulin
反应类型: Sandwich
灵敏度: 25 pg/mL
标准品: 5000 pg/mL
检测范围: 78.1-5000 pg/mL
样本类型: Serum, plasma
反应时长: 1.5h
研究领域: Tags & Cell Markers;Cell Biology;Stem Cells;Cardiovascular
Uniprot ID: P01034
检测原理: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Cys-C, and the Human Cys-C standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human Cys-C. After TMB substrate solution is added, only those wells that contain Human Cys-C and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
5000.00 2.781 2.716
2500.00 1.701 1.637
1250.00 0.906 0.842
625.00 0.508 0.443
312.50 0.319 0.254
156.25 0.177 0.113
78.13 0.120 0.056
0.00 0.064 0.000

精密度

板内精密度(测定内的精密度):CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次,以评估分析板内精密度。

板间精密度(测定板间间精密度):CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次,以评估分析板件的精密度。

回收率

分别往不同样本中添加已知浓度的Cys-C, 做回收率实验,得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 88-99% 94%
EDTA 血浆(n=5) 102-110% 104%
Heparin 血浆(n=5) 98-104% 101%

线性

将添加有Cys-C的样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 93-106% 88-102% 85-101% 96-107%
EDTA 血浆(n=5) 90-103% 94-108% 90-106% 98-105%
Heparin 血浆(n=5) 91-100% 98-107% 92-108% 93-102%

产品成分表

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