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一步法人抗缪勒管激素(AMH)酶联免疫吸附检测试剂盒
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ELK004ES
规格: 价格:
64T ¥1960.00
112T ¥2800.00
112T*5 ¥11900.00

Overview

产品名称: EasyStep Human AMH(Anti-Mullerian Hormone) ELISA Kit
反应性: Human
别名: MIF; MIH; MIS; Müllerian Inhibiting Factor; Müllerian Inhibiting Hormone; Müllerian Inhibiting Substance
反应类型: Sandwich
灵敏度: 0.028 ng/mL
标准品: 20 ng/mL
检测范围: 0.32-20 ng/mL
样本类型: Serum, plasma
反应时长: 1.5h
研究领域: Endocrinology;Reproductive science;Hormone metabolism;
检测原理: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AMH, and the Human AMH standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human AMH. After TMB substrate solution is added, only those wells that contain Human AMH and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AMH in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL) OD Corrected OD
20.00 2.503 2.403
10.00 1.581 1.481
5.00 0.890 0.790
2.50 0.523 0.423
1.25 0.343 0.243
0.63 0.220 0.120
0.32 0.152 0.053
0.00 0.100 0.000

精密度

板内精密度(测定内的精密度):CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次,以评估分析板内精密度。

板间精密度(测定板间间精密度):CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次,以评估分析板件的精密度。

回收率

分别往不同样本中添加已知浓度的AMH, 做回收率实验,得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 90-115% 103%
EDTA 血浆(n=5) 85-106% 98%
Heparin 血浆(n=5) 92-108% 101%

线性

将添加有AMH的样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 80-112% 85-105% 91-110% 93-105%
EDTA 血浆(n=5) 93-113% 86-106% 90-110% 97-103%
Heparin 血浆(n=5) 96-107% 90-103% 88-110% 86-105%

产品成分表

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