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一步法人癌胚抗原(CEA)酶联免疫吸附检测试剂盒
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ELK002ES
规格: 价格:
64T ¥1820.00
112T ¥2600.00
112T*5 ¥11050.00

Overview

产品名称: EasyStep Human CEA(Carcinoembryonic Antigen) ELISA Kit
反应性: Human
别名: CD66E; CD66; CEACAM5; Carcinoembryonic Antigen-related Cell Adhesion Molecule 5; Carcinoembryonic Antigen
反应类型: Sandwich
灵敏度: 7.5 pg/mL
标准品: 10 ng/mL
检测范围: 0.16-10 ng/mL
样本类型: Serum, plasma
反应时长: 1.5h
研究领域: Tumor immunity;Gastroenterology;
Uniprot ID: P06731
检测原理: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CEA, and the Human CEA standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human CEA. After TMB substrate solution is added, only those wells that contain Human CEA and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CEA in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (ng/mL) OD Corrected OD
10.00 3.106 3.055
5.00 2.024 1.973
2.50 1.179 1.129
1.25 0.691 0.641
0.63 0.372 0.322
0.32 0.217 0.167
0.16 0.144 0.094
0.00 0.050 0.000

精密度

板内精密度(测定内的精密度):CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次,以评估分析板内精密度。

板间精密度(测定板间间精密度):CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次,以评估分析板件的精密度。

回收率

分别往不同样本中添加已知浓度的CEA, 做回收率实验,得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 92-110% 101%
EDTA 血浆(n=5) 88-105% 95%
Heparin 血浆(n=5) 90-102% 98%

线性

将添加有CEA的样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 93-105% 85-102% 91-110% 91-108%
EDTA 血浆(n=5) 90-104% 90-106% 88-108% 92-109%
Heparin 血浆(n=5) 88-103% 94-108% 95-105% 88-107%

产品成分表

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