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一步法人白介素6(IL6)酶联免疫吸附检测试剂盒
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ELK001ES
规格: 价格:
64T ¥1680.00
112T ¥2400.00
112T*5 ¥10200.00

Overview

产品名称: EasyStep Human IL6(Interleukin 6) ELISA Kit
反应性: Human
别名: IL-6;MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor
反应类型: Sandwich
灵敏度: 0.1 pg/mL
标准品: 100 pg/mL
检测范围: 1.56-100 pg/mL
样本类型: serum, plasma
反应时长: 1.5 h
研究领域: Cytokine;Tumor immunity;Infection immunity;Cardiovascular biology;
Uniprot ID: P05231
检测原理: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL6, and the Human IL6 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human IL6. After TMB substrate solution is added, only those wells that contain Human IL6 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL6 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

Concentration (pg/mL) OD Corrected OD
100.00 2.999 2.939
50.00 2.373 2.313
25.00 1.683 1.623
12.50 1.117 1.057
6.25 0.708 0.648
3.13 0.344 0.284
1.57 0.175 0.115
0.00 0.060 0.000

精密度

板内精密度(测定内的精密度):CV%<8%

三个已知浓度的样品分别在1个酶标板上测试20次,以评估分析板内精密度。

板间精密度(测定板间间精密度):CV%<10%

三个已知浓度的样品分别在3个不同的酶标板上测试40次,以评估分析板件的精密度。

回收率

分别往不同样本中添加已知浓度的IL6, 做回收率实验,得出回收率范围和平均回收率
样本类型 回收率范围 平均回收率
血清(n=5) 90-105% 97%
EDTA 血浆(n=5) 95-110% 100%
Heparin 血浆(n=5) 93-102% 98%

线性

将添加有IL6的样本分别稀释2倍,4倍,8倍,16倍做回收实验,得出回收率范围
样本类型 1:2 1:4 1:8 1:16
血清(n=5) 85-98% 98-110% 99-116% 95-110%
EDTA 血浆(n=5) 87-102% 85-95% 85-98% 82-95%
Heparin 血浆(n=5) 88-102% 95-106% 95-108% 96-115%

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