|Product name:||TGFb1(TransformingGrowth Factor Beta 1) ELISA Kit|
|Alternative Names:||Cartilage-inducing factor,CED,Differentiation inhibiting factor,DPD1,LAP,Latency-associated peptide,Prepro transforming growth factor beta 1,TGF beta 1,TGF beta,TGF beta 1 protein,TGF-beta 1 protein,TGF-beta-1,TGF-beta-5,TGF-beta1,TGFB,Tgfb-1,tgfb1,TGFB1,TGFbeta,TGFbeta1,Transforming Growth Factor b1,Transforming Growth Factor beta 1,Transforming growth factor beta 1a,transforming growth factor beta-1,transforming growth factor,beta 1,Transforming Growth Factor-ß1|
|Sample Type:||serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids|
|Research Area:||Cytokine;Tumor immunity;Infection immunity;|
|Test principle:||The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TransformingGrowth Factor Beta 1(TGF-b1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TransformingGrowth Factor Beta 1(TGF-b1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TransformingGrowth Factor Beta 1(TGF-b1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TransformingGrowth Factor Beta 1(TGF-b1) in the samples is then determined by comparing the OD of the samples to the standard curve.|
|Concentration (ng/mL)||OD||Corrected OD|
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.