规格: 价格:
48 T ¥1680.00
96 T ¥2400.00


Product name: TGFb1(TransformingGrowth Factor Beta 1) ELISA Kit
Reactivity: General
Alternative Names: Cartilage-inducing factor,CED,Differentiation inhibiting factor,DPD1,LAP,Latency-associated peptide,Prepro transforming growth factor beta 1,TGF beta 1,TGF beta,TGF beta 1 protein,TGF-beta 1 protein,TGF-beta-1,TGF-beta-5,TGF-beta1,TGFB,Tgfb-1,tgfb1,TGFB1,TGFbeta,TGFbeta1,Transforming Growth Factor b1,Transforming Growth Factor beta 1,Transforming growth factor beta 1a,transforming growth factor beta-1,transforming growth factor,beta 1,Transforming Growth Factor-ß1
Assay Type: Sandwich
Sensitivity: 0.10 ng/mL
Standard: 10 ng/mL
Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cytokine;Tumor immunity;Infection immunity;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TransformingGrowth Factor Beta 1(TGF-b1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TransformingGrowth Factor Beta 1(TGF-b1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TransformingGrowth Factor Beta 1(TGF-b1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TransformingGrowth Factor Beta 1(TGF-b1) in the samples is then determined by comparing the OD of the samples to the standard curve.


Concentration (ng/mL) OD Corrected OD
10.00 2.266 2.170
5.00 1.694 1.598
2.50 1.184 1.088
1.25 0.892 0.796
0.63 0.582 0.486
0.32 0.389 0.293
0.16 0.256 0.160
0.00 0.096 0.000


Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.


Matrices listed below were spiked with certain level of recombinant General TGF-b1 and the recovery rates were calculated by comparing the measured value to the expected amount of General TGF-b1 in samples.
Matrix Recovery range Average
serum(n=5) 79-97% 88%
EDTA plasma(n=5) 95-107% 101%
Heparin plasma(n=5) 96-107% 102%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of General TGF-b1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 83-96% 93-102% 86-94% 90-98%
EDTA plasma(n=5) 92-106% 85-97% 91-103% 85-97%
Heparin plasma(n=5) 90-99% 82-101% 88-102% 93-104%


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