小鼠干扰素β(IFNb)酶联免疫吸附检测试剂盒-科鹿（武汉）生物科技有限责任公司

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小鼠干扰素β(IFNb)酶联免疫吸附检测试剂盒

ELK2349

规格：	价格：
48 T	￥1960.00
96 T	￥2800.00

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Overview 文献

Product name:	Mouse IFNb(Interferon Beta) ELISA Kit
Reactivity:	Mouse
Alternative Names:	IFNB1; IFN-B; IFB; IFF; IFNB; Interferon Beta 1 Fibroblast; IFN Beta
Assay Type:	Sandwich
Sensitivity:	2.7 pg/mL
Standard:	500 pg/mL
Range:	7.82-500 pg/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Cytokine;Tumor immunity;Infection immunity;
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Beta(IFNb). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interferon Beta(IFNb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interferon Beta(IFNb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interferon Beta(IFNb) in the samples is then determined by comparing the OD of the samples to the standard curve.

Journal of Neuroimmune Pharmacology

STING/NF-κB/IL-6-mediated inflammation in microglia contributes to spared nerve injury (SNI)-induced neuropathic pain

日期：2021-10-20 IF：7.285
Research Square

STING/NF-κB/IL-6-mediated inflammation in microglia contributes to spared nerve injury (SNI)- induced neuropathic pain

日期：2021-02-09 IF：-

标准曲线

Concentration (pg/mL)	OD	Corrected OD
500.00	1.941	1.853
250.00	1.562	1.474
125.00	0.997	0.909
62.50	0.682	0.594
31.25	0.474	0.386
15.63	0.273	0.185
7.82	0.216	0.128
0.00	0.088	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant IFNb and the recovery rates were calculated by comparing the measured value to the expected amount of IFNb in samples.

Matrix	Recovery range	Average
serum(n=5)	80-97%	88%
EDTA plasma(n=5)	82-94%	88%
Heparin plasma(n=5)	77-95%	86%

线性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFNb and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.